MAP2K3 antibody (pSer189)

Catalog No. ABIN362137

This Rabbit Polyclonal antibody specifically detects MAP2K3 in WB, IHC and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for MAP2K3 antibody (pSer189) (ABIN362137)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MAP2K3 Antibody

Binding Specificity: pSer189

Specificity: The antibody detects endogenous level of MKK3 only when phosphorylated at serine189.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer189 (V-D-S (p) -V-A) derived from Human MKK3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MKK3

Background: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.

Molecular Weight: 40 kDa

NCBI Accession: NP_002747

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2