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MAP2K3 antibody (Mitogen-Activated Protein Kinase Kinase 3) (pSer189)

Details for Product anti-MAP2K3 Antibody No. ABIN362138, Supplier: Login to see
Antigen
  • Mkk3
  • MAPKK3
  • MEK3
  • MKK3
  • PRKMK3
  • SAPKK-2
  • SAPKK2
  • AW212142
  • Prkmk3
  • mMKK3b
  • map2k3
  • zMKK3
Alternatives
anti-Human MAP2K3 antibody for Western Blotting
Epitope
pSer189
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6
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3
3
2
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2
2
1
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Reactivity
Human, Rat (Rattus)
280
108
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17
3
2
1
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Host
Rabbit
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82
Clonality
Polyclonal
Conjugate
This MAP2K3 antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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23
15
8
3
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Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer189 (V-D-S (p) -V-A) derived from Human MKK3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of MKK3 only when phosphorylated at serine189.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MKK3 (MAP2K3 Antibody Abstract)
Background Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
Molecular Weight 40 kDa
NCBI Accession NP_002747
UniProt P46734
Research Area Kinases/Phosphatases
Pathways MAPK Signaling, TLR Signaling
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).