Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) (pSer207) antibody

Details for Product No. ABIN362139
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Antigen
Synonyms MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3, Mkk6, Prkmk6, ANQ1, ARABIDOPSIS NQK1, ARABIDOPSIS THALIANA MAP KINASE KINASE 6, ATMKK6, MAP kinase kinase 6, MIK19.2, MIK19_2, MKK3, map2k3, zMKK3
Epitope
pSer207
(47), (32), (15), (12), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(182), (106), (92), (3), (1)
Host
Rabbit
(177), (24), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(161), (74), (72), (41), (30), (28), (13), (6), (6), (3), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 50 μL
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Immunogen Peptide sequence around phosphorylation site of pSer207 (V-D-S (p) -V-A) derived from Human MKK6. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of MKK6 only when phosphorylated at serine207.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name MKK6 (MAP2K6 Antibody Abstract)
Background Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Molecular Weight 41 kDa
NCBI Accession NP_002749
UniProt P52564
Research Area Kinases/Phosphatases
Pathways MAPK Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).

Validation Images
Catalog No. ABIN362139
207.90 $
Plus shipping costs $45.00
Quantity
Price
50 μL
207.90 $

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