NFKBIA antibody (pSer32, pSer36)

Catalog No. ABIN362151

Cited in 1 publication. The Rabbit Polyclonal anti-NFKBIA antibody (ABIN362151) specifically detects NFKBIA in IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NFKBIA antibody (pSer32, pSer36) (ABIN362151)

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NFKBIA Antibody

Binding Specificity: pSer32, pSer36

Specificity: The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer32, 36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkB-a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-NFKBIA antibody (ABIN362151)

Ren, Cui, Huo, Xue, Cui, Luo, Jiang, Yang: "Cordycepin suppresses TNF-?-induced NF-?B activation by reducing p65 transcriptional activity, inhibiting I?B? phosphorylation, and blocking IKK? ubiquitination." in: International immunopharmacology, Vol. 14, Issue 4, pp. 698-703, (2012) (PubMed).

Target Details for NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Alternative Name: IkappaB-alpha

Background: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.

Molecular Weight: 39 kDa

NCBI Accession: NP_065390

UniProt: P25963

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling