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Tumor Protein P53 (TP53) (pSer32/Ser36) antibody

Antigen

Tumor Protein P53 (TP53)

Synonyms p53, LFS1, TRP53, FLJ92943, P53, Trp53, MGC112612, brp53, drp53, fb40d06, wu:fb40d06, zgc:111919, TP53, bbl, bfy, bhy, p44, Tp53, tp53
Epitope
Alternatives

pSer32/Ser36

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN362151
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name IkappaB-alpha (Phospho-Ser32/Ser36)
Swiss-Prot P04637
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanIkappaB-alpha around the phosphorylation site of serine 32/36 (H-D-S P -G-L-D-S P -M-K).
Isotype IgG  (Matching secondary antibodies)
Specificity IkappaB-alpha (Phospho-Ser32/Ser36) (Human) antibody detects endogenous levels of IkappaB-alpha only whenphosphorylated at serine 32/36.

Application Details

Application Notes IHC: 1:50~1:100 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Tumor Protein P53 (TP53)", type "Antibodies"
Hosts Rabbit (302), Mouse (102), Sheep (3)
Reactivities Human (390), Mouse (Murine) (67), Rat (Rattus) (61), Monkey (5), Cat (Feline) (3), Cow (Bovine) (3), Dog (Canine) (3), Hamster (3), Horse (Equine) (3), Primate (2), Primates (2), Sheep (Ovine) (2), Bird (Avian) (1), Rabbit (1), Xenopus laevis (1)
Applications Western Blotting (WB) (338), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (112), Immunohistochemistry (IHC) (80), Immunofluorescence (IF) (75), ELISA (68), Enzyme Immunoassay (EIA) (68), Immunoprecipitation (IP) (53), Flow Cytometry (FACS) (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Immunocytochemistry (ICC) (11), Dot Blot (Dot) (10), ELISA (Detection) (5), Transfected cell culture (TCC) (3), Chromatin Immunoprecipitation (ChiP) (2), Radioimmunoassay (RIA) (2)
Conjugates FITC (7), Biotin (2), HRP (1), PE (1)
Epitopes pSer315 (12), pSer37 (12), pSer46 (11), pSer33 (10), pThr18 (10), pSer6 (9), pSer392 (8), pSer15 (7), pSer20 (7), AA 371-380 (5), AA 20-25 (4), pSer1232 (4), pSer32/Ser36 (4), AA 18-30 (3), C-Term (3), pSer9 (3), S315 (2), S37 (2), S46 (2), T55 (2), pSer376 (2), pSer378 (2), pThr55 (2), AA 16-30 (1), AA 378-393 (1), AA 46-53 (1), AA 46-55 (1), Acetyl-Lys317 (1), Acetyl-Lys379 (1), S15 (1), S376 (1), S378 (1), S9 (1), T18 (1), acLys120 (1), acLys382 (1), amino acids 211-220 (1), pS20 (1), pS33 (1), phospho (1)