ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (pSer1177)

Details for Product anti-NOS3 Antibody No. ABIN362158, Supplier: Log in to see
Antigen
  • NOS3
  • ECNOS
  • eNOS
  • NOS
  • EC-NOS
  • NOSIII
  • cNOS
  • 2310065A03Rik
  • Nos-3
  • ecNOS
  • eNos
  • ENOS
  • nitric oxide synthase 3 (endothelial cell)
  • endothelial nitric oxide synthase
  • nitric oxide synthase 3, endothelial cell
  • NOS3
  • nos3
  • 23B
  • Nos3
Alternatives
anti-Human ENOS antibody for Immunofluorescence (fixed cells)
Epitope
pSer1177
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This ENOS antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (IHC)
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Options
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Immunogen Peptide sequence around phosphorylation site of pSer1177 (T-Q-S (p) -F-S) derived from Human eNOS. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of eNOS only when phosphorylated at serine 1177.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name eNOS (NOS3 Antibody Abstract)
Background Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Molecular Weight 140 kDa
NCBI Accession NP_000594
UniProt P29474
Research Area Cancer, Enzymes, Metabolism, Inflammation
Pathways ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals
Application Notes Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

Harris, Ju, Venema, Liang, Zou, Michell, Chen, Kemp, Venema: "Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16587-91, 2001 (PubMed).

Wadman: "GM advisory panel is slanted, say critics." in: Nature, Vol. 399, Issue 6731, pp. 7, 1999 (PubMed).