This Phospholipase C gamma 2 antibody is un-conjugated
Application
Western Blotting (WB), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of PLCg2 only when phosphorylated at tyrosine 753.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pTyr753 (S-L-Y (p) -D-V) derived from Human PLCg2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.