Tyrosine Hydroxylase antibody (pSer40)

Catalog No. ABIN362245

This Rabbit Polyclonal antibody specifically detects Tyrosine Hydroxylase in IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Tyrosine Hydroxylase antibody (pSer40) (ABIN362245)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Immunofluorescence (IF)

Product Details anti-Tyrosine Hydroxylase Antibody

Binding Specificity: pSer40

Specificity: The antibody detects endogenous level of Tyrosine Hydroxylase only when phosphorylated at serine 40.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer40 (R-Q-S (p) -L-I) derived from Human pTyrosine Hydroxylase (TH) . Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background: The protein encoded by Tyrosine Hydroxylase is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Molecular Weight: 55-60 kDa

NCBI Accession: NP_000351

UniProt: P07101

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour