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Ribosomal Protein S6 (RPS6) (pSer235) antibody

Antigen

Ribosomal Protein S6 (RPS6)

Epitope
Alternatives

pSer235

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Application
Alternatives Western Blotting (WB), Immunofluorescence (IF)
Catalog no. ABIN362281
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name S6 Ribosomal Protein (Phospho-Ser235)
Swiss-Prot P62753
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human S6Ribosomal protein around the phosphorylation site of serine 235 (R-L-S P -S-L).
Isotype IgG  (Matching secondary antibodies)
Specificity S6 Ribosomal Protein (Phospho-Ser235) (Human) antibody detects endogenous levels of S6Ribosomal protein only when phosphorylated at serine 235.

Application Details

Application Notes WB: 1:500~1:1000 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Ribosomal Protein S6 (RPS6)", type "Antibodies"
Hosts Rabbit (66), Mouse (3)
Reactivities Human (64), Mouse (Murine) (43), Rat (Rattus) (43), Chicken (4), Dog (Canine) (4), Horse (Equine) (4), Rabbit (4), Cow (Bovine) (3), Pig (Porcine) (3), Guinea Pig (2)
Applications Western Blotting (WB) (42), Immunofluorescence (IF) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Flow Cytometry (FACS) (19), ELISA (8), Enzyme Immunoassay (EIA) (7), Immunohistochemistry (IHC) (7), Immunoprecipitation (IP) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer235 (7), pSer235/236 (6), C-Term (2), N-Term (2)