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H2A Histone Family, Member X (H2AFX) (pSer139) antibody

Antigen

H2A Histone Family, Member X (H2AFX)
Synonyms H2AX, H2A.X, H2A/X, H2ax, AW228881, Hist5-2ax, h2afx, MGC82078
Epitope
Alternatives

pSer139
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Application
Alternatives Western Blotting (WB), Immunofluorescence (IF)
Catalog no. ABIN362339
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Histone H2A.X (Phospho-Ser139)
Swiss-Prot P16104
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanHistone H2A.X around the phosphorylation site of serine 139 (Q-A-S P -Q-E).
Isotype IgG  (Matching secondary antibodies)
Specificity Histone H2A.X (Phospho-Ser139) (Human) antibody detects endogenous levels of Histone H2A.Xonly when phosphorylated at serine 139.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes WB: 1:500~1:1000 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "H2A Histone Family, Member X (H2AFX)", type "Antibodies"
Hosts Rabbit (63), Mouse (4)
Reactivities Human (64), Mouse (Murine) (33), Rat (Rattus) (33), Cow (Bovine) (18), Dog (Canine) (17), Horse (Equine) (17), Rabbit (16), Pig (Porcine) (15), Chicken (3), Monkey (1)
Applications Western Blotting (WB) (47), Immunofluorescence (IF) (39), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (12), Immunohistochemistry (IHC) (10), ELISA (8), Immunoprecipitation (IP) (6), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer139 (11)