H2AFX antibody (pSer139)

Catalog No. ABIN6971765

This Rabbit Polyclonal antibody specifically detects H2AFX in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for H2AFX antibody (pSer139) (ABIN6971765)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Characteristics: Histone H2AX phospho Ser139 (H2AX, H2A histone family member X) replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. Histones thereby play a central role in transcriptional regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called the histone code, and nucleosome remodeling. Histone H2AX is required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation, and for efficient repair of DNA double-strand breaks (DSBs), specifically when modified by C-terminal phosphorylation. Histone H2A.XS139ph antibody (pAb) (H2AX) was raised in a Rabbit host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.

Purification: Protein A Chromatography

Immunogen: This Histone H2AX phospho Ser139 antibody was raised against a peptide including phosphoserine 139 of histone H2AX.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2A.X

Molecular Weight: 15 kDa

NCBI Accession: NP_002096

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus