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Antibodies
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr291) antibody
Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr291) antibody
| Antigen |
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| Synonyms |
MEK1, MKK1, MAPKK1, PRKMK1, Mek1, MEKK1, Prkmk1 |
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Epitope
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pThr291 (8), pSer221 (7), pSer217 (5), pSer218/222 (5), pThr292 (4), N-Term (3), Center (2), S297 (2), T291 (2), T385 (2), pS217 (2), pSer217/Ser221 (2), pSer297 (2), pThr385 (2), S218 (1), S218/222 (1), pS218 (1), pS222 (1), pSer217, pSer221 (1), pSer217/221 (1), pSer218 (1), pSer222 (1)
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN362381 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MEK1 (Phospho-Thr291)
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Swiss-Prot
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Q02750
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Immunogen
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The antiserum was produced against synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291 (P-R-T P -P-G).
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Isotype
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IgG (Matching secondary antibodies)
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Specificity
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MEK1 (Phospho-Thr291) (Human) antibody detects endogenous levels of MEK1 only when phosphorylated at threonine 291.
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Application Notes
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WB: 1:500~1:1000
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Concentration
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50µg/50µl
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage
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Store at -20°C. Avoid repeat freeze-thaw cycles.
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Restrictions
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For Research Use only
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Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)", type "Antibodies"
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Hosts
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Rabbit (134), Mouse (8)
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Reactivities
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Human (136), Mouse (Murine) (69), Rat (Rattus) (64), Dog (Canine) (10), Cow (Bovine) (9), Xenopus laevis (7), Monkey (5), Chicken (4), Hamster (2), Zebrafish (Danio rerio) (1)
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Applications
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Western Blotting (WB) (111), Immunohistochemistry (IHC) (43), Enzyme Immunoassay (EIA) (39), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (33), Dot Blot (Dot) (18), ELISA (17), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Flow Cytometry (FACS) (1)
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Epitopes
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pThr291 (8), pSer221 (7), pSer217 (5), pSer218/222 (5), pThr292 (4), N-Term (3), Center (2), S297 (2), T291 (2), T385 (2), pS217 (2), pSer217/Ser221 (2), pSer297 (2), pThr385 (2), S218 (1), S218/222 (1), pS218 (1), pS222 (1), pSer217, pSer221 (1), pSer217/221 (1), pSer218 (1), pSer222 (1)
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Alternatives 
