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SH2 Domain Protein C1 (SHC1) (pTyr427) antibody

Antigen

SH2 Domain Protein C1 (SHC1)
Synonyms SHC, SHCA, FLJ26504, P66shc, shc, MGC98812
Epitope
Alternatives

pTyr427
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Catalog no. ABIN362405
Quantity 50µg  (50µg/50µl)  (Variants)
Price 280.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Shc1 (Phospho-Tyr427)
Swiss-Prot P29353
Immunogen The antiserum was produced against synthesized phosphopeptide derived from humanShc1 around the phosphorylation site of tyrosine 427 (P-S-Y P -V-N).
Isotype IgG  (Matching secondary antibodies)
Specificity Shc1 (Phospho-Tyr427) (Human) antibody detects endogenous levels of Shc1 only whenphosphorylated at tyrosine 427.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 IF:1:100~1:200
Concentration 50µg/50µl
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "SH2 Domain Protein C1 (SHC1)", type "Antibodies"
Hosts Rabbit (51), Mouse (4)
Reactivities Human (54), Mouse (Murine) (39), Rat (Rattus) (34), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2), Chicken (1), Horse (Equine) (1)
Applications Western Blotting (WB) (37), Immunofluorescence (IF) (34), Flow Cytometry (FACS) (20), Immunohistochemistry (IHC) (15), ELISA (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Proximity Ligation Assay (PLA) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pTyr349 (4), pTyr427 (3), Center (1), Y427 (1), pY427 (1)