ERBB3 antibody (pTyr1328)
Quick Overview for ERBB3 antibody (pTyr1328) (ABIN362443)
Target
See all ERBB3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pTyr1328
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Specificity
- The antibody detects endogenous level of Her3/ErbB3 only when phosphorylated at tyrosine 1328.
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Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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Immunogen
- Peptide sequence around phosphorylation site of pTyr1328 (P-D-Y (p) -W-H) derived from Human Her3, ErbB3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
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Isotype
- IgG
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Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))
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Alternative Name
- Her3/ErbB3
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Background
- Binds and is activated by neuregulins and NTAK.
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Molecular Weight
- 185 kDa
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NCBI Accession
- NP_001005
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UniProt
- P21860
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Target
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