EIF4B antibody (pSer422)

Catalog No. ABIN362448

Product Details anti-EIF4B Antibody

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Binding Specificity: pSer422

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: The antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer422 (T-G-S (p) -E-S) derived from Human eIF4B. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eIF4B (EIF4B Products)

Synonyms: EIF-4B antibody, PRO1843 antibody, CG10837 antibody, Dm-eIF4B antibody, Dmel\\CG10837 antibody, Eif4B antibody, eIF-4B-L antibody, eIF-4B-S antibody, eIF-4b antibody, eIF4B antibody, eIF4B-L antibody, eIF4B-S antibody, eIf4b antibody, 2310046H11Rik antibody, AL024095 antibody, C85189 antibody, Eif4a2 antibody, eif4b antibody, fb15c09 antibody, wu:fb15c09 antibody, zgc:101532 antibody, MGC89384 antibody, eukaryotic translation initiation factor 4B antibody, eukaryotic initiation factor 4B antibody, Eukaryotic initiation factor 4B antibody, eukaryotic translation initiation factor 4Bb antibody, eukaryotic translation initiation factor 4Ba antibody, eukaryotic translation initiation factor 4B S homeolog antibody, EIF4B antibody, Eif4B antibody, eIF-4B antibody, Eif4b antibody, eif4bb antibody, eif4b antibody, eif4ba antibody, eif4b.S antibody, LOC100284982 antibody, LOC100380867 antibody

Background: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Molecular Weight: 80 kDa

NCBI Accession: NP_001408

UniProt: P23588