CDK6 antibody (pTyr13)

Catalog No. ABIN362471

The Rabbit Polyclonal anti-CDK6 antibody is suitable to detect CDK6 in samples from Human and Mouse. It has been validated for IHC and IF.

Quick Overview for CDK6 antibody (pTyr13) (ABIN362471)

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDK6 Antibody

Binding Specificity: pTyr13

Specificity: The antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 13.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr13 (Q-Q-Y (p) -E-C) derived from Human CDK6. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for CDK6

Target: CDK6 (Cyclin-Dependent Kinase 6 (CDK6))

Alternative Name: CDK6

Background: Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.

Molecular Weight: 36 kDa

NCBI Accession: NP_001138

UniProt: Q00534

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases