Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2) (AA 392-396) antibody

Details for Product No. ABIN362497
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Antigen
Synonyms CFC4, MAPKK2, MEK2, MKK2, PRKMK2, AA589381, MK2, Prkmk2
Epitope
AA 392-396
(37), (12), (8), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(140), (93), (85), (13), (4), (3), (1)
Host
Rabbit
(121), (24)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(113), (45), (40), (31), (30), (24), (22), (8), (7), (4), (2), (2), (1), (1)
Pubmed 4 references available
Catalog no. ABIN362497
Quantity 50 µL
Price
132.00 $   Plus shipping costs $45.00
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Immunogen Peptide sequence around AA 392-396 (P-G-T-P-T) derived from Human MEK-2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total MEK2 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative Name MEK-2
Background Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases
Molecular Weight 44 kDa
NCBI Accession NP_109587
UniProt P36507
Research Area Cancer, Tyrosine Kinases, Kinases/Phosphatases
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Cowley, Paterson, Kemp et al.: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, 1994 (PubMed).

Rosen, Ginty, Weber et al.: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, 1994 (PubMed).

Alessi, Saito, Campbell et al.: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, 1994 (PubMed).

Hosts (121), (24)
Reactivities (140), (93), (85), (13), (4), (3), (1)
Applications (113), (45), (40), (31), (30), (24), (22), (8), (7), (4), (2), (2), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (37), (12), (8), (6), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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