IGF1R antibody (AA 1159-1163)

Catalog No. ABIN362636

This Rabbit Polyclonal antibody specifically detects IGF1R in WB, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 2+ publications

Quick Overview for IGF1R antibody (AA 1159-1163) (ABIN362636)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-IGF1R Antibody

Binding Specificity: AA 1159-1163

Specificity: The antibody detects endogenous level of total IGF-1R protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 1159-1163 (D-I-Y-E-T) derived from Human IGF-1R . Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-IGF1R antibody (ABIN362636)

Haghir, Rezaee, Nomani, Sankian, Kheradmand, Hami: "Sexual dimorphism in expression of insulin and insulin-like growth factor-I receptors in developing rat cerebellum." in: Cellular and molecular neurobiology, Vol. 33, Issue 3, pp. 369-77, (2013) (PubMed).

Yamamoto, Oshima, Yoshihara, Nishi, Arai, Inui, Kaneko, Nozawa, Adachi, Rino, Masuda, Imada: "Clinical significance of immunohistochemical expression of insulin-like growth factor-1 receptor and matrix metalloproteinase-7 in resected non-small cell lung cancer." in: Experimental and therapeutic medicine, Vol. 3, Issue 5, pp. 797-802, (2012) (PubMed).

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: IGF-1R

Background: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.

Molecular Weight: 95 kDa

NCBI Accession: NP_000866

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy