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Histone H3.1 (HIST1H3B) (AA 8-12) antibody

Details for Product No. ABIN362735
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Synonyms H3.f, H3/f, H3FF, H3-H, H3 histone, Hist1h2ai, H3/i, H3FI, H3.1-221, M32460
AA 8-12
(30), (15), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(82), (46), (43), (1), (1), (1), (1)
(74), (9)
(2), (2), (2), (2), (2), (2)
Western Blotting (WB), Immunofluorescence (IF)
(73), (46), (40), (15), (12), (4)
Pubmed 4 references available
Quantity 50 µL
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Catalog No. ABIN362735
165.00 $
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Immunogen Peptide sequence around AA 8-12 (R-K-S-T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total Histone H3.1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name Histone H3.1
Background Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Molecular Weight 17 kDa
NCBI Accession NP_003521
UniProt P68431
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Workman, Kingston: "Alteration of nucleosome structure as a mechanism of transcriptional regulation." in: Annual review of biochemistry, Vol. 67, pp. 545-79, 1998 (PubMed).

Hansen, Tse, Wolffe: "Structure and function of the core histone N-termini: more than meets the eye." in: Biochemistry, Vol. 37, Issue 51, pp. 17637-41, 1999 (PubMed).

"Resolutions to enhance confident creativity." in: Nature, Vol. 403, Issue 6765, pp. 1, 2000 (PubMed).

Cheung, Allis, Sassone-Corsi: "Signaling to chromatin through histone modifications." in: Cell, Vol. 103, Issue 2, pp. 263-71, 2000 (PubMed).

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