Histone H3.1 antibody (pSer10)

Catalog No. ABIN1531863

The Rabbit Polyclonal anti-Histone H3.1 antibody (ABIN1531863) specifically detects Histone H3.1 in WB, IF, ELISA, IHC, ICC, FACS and IHC (fro). The antibody is reactive with Human, Mouse, Rat and Drosophila melanogaster samples.

Quick Overview for Histone H3.1 antibody (pSer10) (ABIN1531863)

Target: Histone H3.1 (HIST1H3B)

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Histone H3.1 Antibody

Binding Specificity: AA 1-50, pSer10

Purpose: Histone H3 (phospho Ser10) Antibody

Specificity: Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ICC:IF 1:200 - 1:1000. ELISA: 1:10000. IHC-f,Flow Cyt 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1

Background:

Synonyms: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Gene Name: HIST1H3A

Protein Name: Histone H3.1

Molecular Weight: 15 kDa

Gene ID: 8350, 8351, 8352

OMIM: 602810

UniProt: P68431