Did you know that you can buy products from over 140 different suppliers from us?

Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6) (AA 205-209) antibody

Details for Product No. ABIN362760
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3, Mkk6, Prkmk6, ANQ1, ARABIDOPSIS NQK1, ARABIDOPSIS THALIANA MAP KINASE KINASE 6, ATMKK6, MAP kinase kinase 6, MIK19.2, MIK19_2, MKK3, map2k3, zMKK3
Epitope
AA 205-209
(38), (19), (13), (8), (7), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(147), (91), (81), (12), (12), (12), (1), (1)
Host
Rabbit
(129), (28), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(125), (69), (50), (30), (29), (22), (17), (8), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 50 µL
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN362760
165.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide sequence around AA 205-209 (V-D-S-V-A) derived from Human MKK6. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total MKK6 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name MKK6
Background MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
Molecular Weight 41 kDa
NCBI Accession NP_002749
UniProt P52564
Research Area Kinases/Phosphatases
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).

Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Validation Images
back to top