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ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (AA 1175-1179)

Details for Product anti-NOS3 Antibody No. ABIN362790, Supplier: Log in to see
Antigen
  • 2310065A03Rik
  • cNOS
  • EC-NOS
  • ecNOS
  • ECNOS
  • eNOS
  • eNos
  • ENOS
  • NOS
  • Nos-3
  • NOS3
  • NOSIII
Alternatives
anti-Human ENOS antibody for Enzyme Immunoassay
Epitope
AA 1175-1179
51
24
24
22
21
18
16
15
13
13
13
13
9
9
9
8
6
6
6
5
5
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
389
218
204
33
33
11
11
11
2
2
2
2
2
1
1
1
1
Host
Rabbit
419
41
Clonality
Polyclonal
Conjugate
This ENOS antibody is un-conjugated
28
25
17
7
7
6
6
6
6
6
6
6
6
6
6
Application
Immunofluorescence (IF), Western Blotting (WB)
297
176
68
66
62
59
24
24
23
19
11
9
5
2
2
1
1
Supplier
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Immunogen Peptide sequence around AA 1175-1179 (T-Q-S-F-S) derived from Human eNOS. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total eNOS protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Alternative Name eNOS (NOS3 Antibody Abstract)
Background Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Molecular Weight 140 kDa
NCBI Accession NP_000594
UniProt P29474
Research Area Cancer, Enzymes, Metabolism, Inflammation
Pathways ACE Inhibitor Pathway
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Jiao, Wang, Lou, Jin, Fan, Li, Han, Zhang: "Regulation of ciliary beat frequency by the nitric oxide signaling pathway in mouse nasal and tracheal epithelial cells." in: Experimental cell research, Vol. 317, Issue 17, pp. 2548-53, 2011 (PubMed).

Background publications Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

Harris, Ju, Venema, Liang, Zou, Michell, Chen, Kemp, Venema: "Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16587-91, 2001 (PubMed).

Wadman: "GM advisory panel is slanted, say critics." in: Nature, Vol. 399, Issue 6731, pp. 7, 1999 (PubMed).