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PI3K P85 alpha/gamma/beta (AA 465-469) antibody

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AA 465-469
Human, Mouse (Murine), Rat (Rattus)
Immunohistochemistry (IHC)
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Immunogen Peptide sequence around AA 465-469, 197-201, 462-466 (L-Y-E-E-Y) derived from Human PI3K P85. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total PI3K P85 a/g/b protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Background PI3K binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Molecular Weight 85 kDa
UniProt O00459
Application Notes Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Li, Lu, Chen, Bu, Huang: "Loss of fatty acid synthase inhibits the "HER2-PI3K/Akt axis" activity and malignant phenotype of Caco-2 cells." in: Lipids in health and disease, Vol. 12, pp. 83, 2013

Li, Bu, Wu, Wu, Huang: "The "HER2-PI3K/Akt-FASN Axis" regulated malignant phenotype of colorectal cancer cells." in: Lipids, Vol. 47, Issue 4, pp. 403-11, 2012

Background publications Neri, Borgatti, Capitani, Martelli: "The nuclear phosphoinositide 3-kinase/AKT pathway: a new second messenger system." in: Biochimica et biophysica acta, Vol. 1584, Issue 2-3, pp. 73-80, 2002

Simpson, Parsons: "PTEN: life as a tumor suppressor." in: Experimental cell research, Vol. 264, Issue 1, pp. 29-41, 2001

Stoyanov, Volinia, Hanck, Rubio, Loubtchenkov, Malek, Stoyanova, Vanhaesebroeck, Dhand, Nürnberg: "Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase." in: Science (New York, N.Y.), Vol. 269, Issue 5224, pp. 690-3, 1995