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Mouse anti-Human IgE (Fc Region) Antibody

Details for Product No. ABIN363694, Supplier: Log in to see
Antigen
Epitope
Fc Region
87
65
40
8
7
2
1
1
Reactivity
Human
369
102
35
28
5
3
2
2
2
2
1
1
1
Host
Mouse
220
206
58
38
5
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
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Immunogen purified human myeloma IgE
Clone Le27-NBS01
Isotype IgG1 kappa
Specificity Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
Characteristics IGHE, chromosome 14q32.33
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Purity > 95 %, tested by analytical Gel Permeation Chromatography
Sterility Sterile filtered
Background The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
Research Area Immunology, Secondary Antibodies
Application Notes For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, pH=7.2, sterile filtered, contains no NaN3
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C
Product cited in: Rudolf, Zuercher, Nechansky et al.: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

Background publications Knutti-Mueller, Stadler, Magnusson et al.: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).