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Details for Product No. ABIN363695

Immunoglobulin E (IgE) (Fc specific) antibody

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Antigen
Epitope
Fc specific
Reactivity
Human
Host
Mouse
Clonality (Clone) Monoclonal ()
Application
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
Pubmed 2 references available
Catalog no. ABIN363695
Quantity 500 µg
Price
551.76 $   Plus shipping costs $45.00
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Availability Will be delivered in 4 to 5 Business Days
Immunogen purified human myeloma IgE
Clone Le27-NBS01
Isotype IgG1 kappa
Specificity Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
Purification Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Purity > 95%, tested by analytical Gel Permeation Chromatography
Sterility Sterile filtered
Alternative Name Immunoglobulin E (IgE) (Fc)
Background The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
Gene ID IGHE, chromosome 14q32.33
Research Area Allergy
Application Notes For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100 Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions For Research Use only
Concentration 1mg/ml
Buffer PBS, pH=7.2, sterile filtered, contains no NaN3
Preservative Azide free
Storage 4 °C
Knutti-Mueller, Stadler, Magnusson et al.: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).

Rudolf, Zuercher, Nechansky et al.: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

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