PAFAH1B1 antibody (AA 397-410)

Catalog No. ABIN364420

Product Details anti-PAFAH1B1 Antibody

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Binding Specificity: AA 397-410

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Horse, Pig, Rabbit, Chicken, Xenopus laevis, Hamster, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This PAFAH1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PAFAH1B1 / LIS1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Water flea, Tick (100%) Chimpanzee, Drosophila, Ant, Capsaspora (93%) Catfish, Mosquito, Sea anemone, Beetle, Trichoplax (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide TGSVDQTVKVWECR from the C-terminus of human PAFAH1B1 / LIS1 (NP_000421.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Cat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Salmon, Stickleback, Pufferfish, Zebrafish, Water flea, Tick (100%), Chimpanzee, Drosophila, Ant, Capsaspora (93%), Catfish, Mosquito, Sea anemone, Beetle, Trichoplax (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PAFAH1B1

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Alternative Name: PAFAH1B1 / LIS1 (PAFAH1B1 Products)

Synonyms: LIS1 antibody, LIS2 antibody, MDCR antibody, MDS antibody, PAFAH antibody, LIS-1 antibody, Lis1 antibody, MMS10-U antibody, Mdsh antibody, Ms10u antibody, Pafaha antibody, lis2 antibody, mdcr antibody, mds antibody, pafah antibody, pafah1b1-b antibody, PAFAHA antibody, Lis1a antibody, platelet activating factor acetylhydrolase 1b regulatory subunit 1 antibody, platelet-activating factor acetylhydrolase, isoform 1b, subunit 1 antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 antibody, platelet activating factor acetylhydrolase 1b regulatory subunit 1 L homeolog antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1a antibody, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1b antibody, PAFAH1B1 antibody, Pafah1b1 antibody, pafah1b1.L antibody, pafah1b1a antibody, pafah1b1b antibody

Background: Name/Gene ID: PAFAH1B1

Synonyms: PAFAH1B1, LIS-1, LIS2, Lissencephaly 1 protein, Lissencephaly-1 protein, MDCR, MDS, PAFAHA, PAF-AH alpha, PAFAH, LIS1, PAF-AH 45 kDa subunit, PAFAH alpha

Gene ID: 5048

NCBI Accession: NP_000421

UniProt: P43034

Pathways: M Phase, Regulation of Cell Size