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T-Cell Receptor gamma/delta antibody (FITC)

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Antigen
Reactivity
Mouse (Murine)
115
54
48
16
2
1
1
Host
Hamster
118
91
27
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
42
38
21
8
6
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
Application
Flow Cytometry (FACS)
215
96
54
51
14
13
9
8
6
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
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Clone UC7-13D5
Isotype IgG
Characteristics Synonyms: TCRG, TCRD, T-Cell Receptor gamma, T-Cell Receptor delta, T-Cell Receptor gamma delta
Alternative Name T Cell Receptor / TCR gamma/delta
Background T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic and consists of Ig-like variable and constant domains. (Ref.1) It is expressed during thymopoiesis and on the small subpopulation of gamma/delta TCR expressing T lymphocytes in the periphery. The majority of T cells present in some epithelial tissues are gamma/delta+ and have limited receptor diversity. (Ref.1,2) The TCR gamma/delta heterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signal transduction mediated by the invariant chains leads to T cell activation. (Ref.3)Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCRD, TCRG
Research Area Immunology, Receptors, Signaling
Application Notes Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.4-7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Barrett, Hedrick, Dent, Delvy, Kennedy, Matis, Bluestone: "Control of self-reactivity in the intestine." in: Immunologic research, Vol. 10, Issue 3-4, pp. 341-8, 1992 (PubMed).

Allison, Havran: "The immunobiology of T cells with invariant gamma delta antigen receptors." in: Annual review of immunology, Vol. 9, pp. 679-705, 1991 (PubMed).

Klausner, Samelson: "T cell antigen receptor activation pathways: the tyrosine kinase connection." in: Cell, Vol. 64, Issue 5, pp. 875-8, 1991 (PubMed).

Bluestone, Cron, Barrett, Houlden, Sperling, Dent, Hedrick, Rellahan, Matis: "Repertoire development and ligand specificity of murine TCR gamma delta cells." in: Immunological reviews, Vol. 120, pp. 5-33, 1991 (PubMed).

Davis: "T cell receptor gene diversity and selection." in: Annual review of biochemistry, Vol. 59, pp. 475-96, 1990 (PubMed).

Dent, Matis, Hooshmand, Widacki, Bluestone, Hedrick: "Self-reactive gamma delta T cells are eliminated in the thymus." in: Nature, Vol. 343, Issue 6260, pp. 714-9, 1990 (PubMed).

Houlden, Matis, Cron, Widacki, Brown, Pampeno, Meruelo, Bluestone: "A TCR gamma delta cell recognizing a novel TL-encoded gene product." in: Cold Spring Harbor symposia on quantitative biology, Vol. 54 Pt 1, pp. 45-55, 1990 (PubMed).