This antibody HBF-719 reacts with the B6 allele of Ly49F. It stains ~ 11 % of NK1.1+CD3- splenocytes, and 3.5 % (2.5 month old) to 10.0 % (11 month old) of CD8+ spleen cells from C57BL/6 mice. (Ref.1)
Flow Cytometry: < / = 1 μg/10^6 cells. (Ref.1,3) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
Klra6
(Killer Cell Lectin-Like Receptor, Subfamily A, Member 6 (Klra6))
Ly49f antibody, killer cell lectin-like receptor, subfamily A, member 6 antibody, killer cell lectin-like receptor antibody, Klra6 antibody, LY49F antibody
Background
Ly49F is a member of the lectin like homodimeric Ly49 family of cell surface receptors. (Ref.1) Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down regulated. (Ref.2) These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells.Synonyms: Killer cell lectin-like receptor 6, Ly-49f, Ly49-f, Ly49F, Lymphocyte antigen 49f, T-cell surface glycoprotein Ly-49F