CD3 antibody

Catalog No. ABIN371285

Product Details anti-CD3 Antibody

Target: CD3

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: This antibody recognizes Porcine CD3e (Mr. 20 kDa). The Monoclonal antibody PPT3 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1)

Characteristics: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Purification: Purified

Clone: PPT3

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). Immunoprecipitation In vitro activation of T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Synonyms: CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 397455

UniProt: Q7YRN2

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway