Bu-1b antibody

Catalog No. ABIN371415

Product Details anti-Bu-1b Antibody

Target: Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bu-1b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Characteristics: Synonyms: B6.2, Bu1b

Purification: Purified

Immunogen: Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.

Clone: 5-11G2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Immunoprecipitation. Immunohistochemistry on Frozen Sections. (Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Bu-1b

Target: Bu-1b (Bu-1b (B Cell Surface Alloantigens) (Bu-1b))

Alternative Name: Bu-1b (Bu-1b Products)

Synonyms: B6.2 antibody, Bu-1 antibody, Bu-1b antibody, B6.1 antibody, LOC396098 antibody

Background: Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90 % of bursal cells, 2 to 8 % of thymocytes, 15 to 27 % of spleen cells, and 2 to 18 % of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.Synonyms: B6.2, chicken B-cell marker

UniProt: Q90747