INA antibody

Catalog No. ABIN371820

Product Details anti-INA Antibody

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~66k alpha Internexin protein.

Cross-Reactivity (Details): Species reactivity (tested):most Mammalian.

Characteristics: Synonyms: Alpha-Inx, INA, NEF5, NF66, 66 kDa neurofilament protein, Neurofilament-66

Purification: Ig Fraction

Immunogen: Recombinant rat alpha-Internexin expressed in and purified from E. coli.

Clone: ID2

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1/1,000. This antibody can be used on formalin-fixed cells in tissue culture, cryostat sections. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of theprotein and is unusually resistant to aldehyde fixation, so that this antibody is ideal forstudies of paraffin embedded formalin fixed histological sections. Western blotting: 1/2,000. Immunofluorescence: 1/250.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: alpha-Internexin (INA Products)

Synonyms: NEF5 antibody, NF-66 antibody, TXBP-1 antibody, Inexa antibody, Intlaa antibody, Nf66 antibody, AV028420 antibody, NF66 antibody, ina-A antibody, xefiltin antibody, ina-a antibody, inexa antibody, MGC82496 antibody, INA antibody, si:ch211-67n3.6 antibody, nef5 antibody, xina antibody, nf-66 antibody, txbp-1 antibody, MGC69277 antibody, gefiltin antibody, zgc:110516 antibody, internexin neuronal intermediate filament protein alpha antibody, internexin neuronal intermediate filament protein, alpha antibody, internexin neuronal intermediate filament protein alpha S homeolog antibody, low molecular weight neuronal intermediate filament S homeolog antibody, internexin neuronal intermediate filament protein, alpha a antibody, internexin neuronal intermediate filament protein, alpha b antibody, INA antibody, Ina antibody, ina.S antibody, nif.S antibody, inaa antibody, ina antibody, inab antibody

Background: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).Synonyms: 66 kDa neurofilament protein, Alpha-Inx, INA, NEF5, NF66, Neurofilament-66

Gene ID: 9118

UniProt: Q16352