INA antibody (AA 71-161)

Catalog No. ABIN4886634

The Rabbit Polyclonal anti-INA antibody (ABIN4886634) specifically detects INA in WB, IHC, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for INA antibody (AA 71-161) (ABIN4886634)

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-INA Antibody

Binding Specificity: AA 71-161

Purpose: Anti-Alpha Internexin/INA Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse,rat Alpha Internexin.

Characteristics: Anti-Alpha Internexin/INA Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Alpha Internexin recombinant protein (Position: A71-R161). Human Alpha Internexin shares 98.9% amino acid (aa) sequence identity with both mouse and rat Alpha Internexin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for INA

Target: INA (Internexin Neuronal Intermediate Filament Protein, alpha (INA))

Alternative Name: INA

Background:

Background: Alpha-Internexin (INA, also NF-66) is a 66 kDa member of the intermediate filament (IF) protein family. The protein was originally purified from rat optic nerve and spinal cord. And the protein copurifies with other neurofilament subunits, as it was originally discovered, however in some mature neurons it can be the only neurofilament expressed. The protein is present in developing neuroblasts and in the Central Nervous System of adults. Meanwhile, the protein is a major component of the intermediate filament network in small interneurons and cerebellar granule cells, where it is present in the parallel fibers.

Gene Full Name: internexin neuronal intermediate filament protein alpha

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 62-66 kDa

Gene ID: 9118

UniProt: Q16352