Immunohistochemistry on Paraffin Sections: 10 μg/mL. Western blot: 1-3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
RANK is a member of the tumor necrosis factor receptor (TNFR) family. Human and murine RANK share 81 % amino acid identity in their extracellular domains. RANK is widely expressed, with highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is also expressed in dendritic cells. RANK and RANK ligand (RANKL) are important regulators of interactions between T cells and dendritic cells. RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand, osteoprotegerin ligand and osteoclast differentiation factor) is the ligand for RANK. The biological functions mediated by RANK include activation of NFkappaB and cjun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis and lymph node organogenesis. The soluble form of RANK is able to block TRANCE induced biological activity. The binding of anti-RANK to cell surface RANK triggers signal transduction and induces RANK mediated bioactivity.Synonyms: ODFR, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, TNFRSF11A, Tumor necrosis factor receptor superfamily member 11A