INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Reactivity
Human, Mouse, Rat
Host
Chicken
Clonality
Polyclonal
Conjugate
This INA antibody is un-conjugated
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
Specific for the ~66k alpha Internexin protein. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent Alpha-Internexin breakdown products.
Cross-Reactivity (Details)
Species reactivity (expected):many other Mammals. Species reactivity (tested):Human, Mouse and Rat.
Purification
Ig Fraction
Immunogen
Recombinant rat alpha-internexin expressed in and purified from E. coli.
INA
Reactivity: Human, Mouse
WB
Host: Mouse
Monoclonal
7T2
unconjugated
Application Notes
Western Blot: 1/1000. Immunofluorescence: 1/500. Immunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Target
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).Synonyms: 66 kDa neurofilament protein, Alpha-Inx, INA, NEF5, NF66, Neurofilament-66