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CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (pThr286)

Details for Product anti-CAMK2A Antibody No. ABIN372594, Supplier: Log in to see
Antigen
  • camk2
  • camk2b
  • CAMKA
  • CaMKII
  • mKIAA0968
  • PK2CDD
  • PKCCD
  • R74975
  • zgc:112538
  • zgc:123320
Alternatives
anti-Human CAMK2A antibody for Western Blotting
Epitope
pThr286
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8
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Reactivity
Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
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10
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This CAMK2A antibody is un-conjugated
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6
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Application
Western Blotting (WB)
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55
43
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22
9
7
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1
Supplier
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.
Isotype IgG
Specificity Specific for the ~50k Alpha-CaM Kinase II and the ~60k Beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the lambda-phosphatase treatment.
Cross-Reactivity (Details) Species reactivity (expected):Human, Mouse and Xenopus.
Species reactivity (tested):Rat.
Purification Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular responses to a wide variety of intercellular signals (Kennedy, 1998, Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003, Soderling, 2000, Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003, Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning (Elgersma et al., 2002).Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II, KIAA0968
Gene ID 815
NCBI Accession NP_741960
UniProt Q9UQM7
Research Area Kinases/Phosphatases
Pathways WNT Signaling, Interferon-gamma Pathway
Application Notes Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.
Supplier Images
 image for anti-CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (pThr286) (ABIN372594) anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286) antibody
Product cited in: Magupalli, Mochida, Yan, Jiang, Westenbroek, Nairn, Scheuer, Catterall: "Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 4637-48, 2013

Kothmann, Trexler, Whitaker, Li, Massey, OBrien: "Nonsynaptic NMDA receptors mediate activity-dependent plasticity of gap junctional coupling in the AII amacrine cell network." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 20, pp. 6747-59, 2012

Coultrap, Buard, Kulbe, DellAcqua, Bayer: "CaMKII autonomy is substrate-dependent and further stimulated by Ca2+/calmodulin." in: The Journal of biological chemistry, Vol. 285, Issue 23, pp. 17930-7, 2010

Vest, OLeary, Coultrap, Kindy, Bayer: "Effective post-insult neuroprotection by a novel Ca(2+)/ calmodulin-dependent protein kinase II (CaMKII) inhibitor." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20675-82, 2010

Sun, Milovanovic, Zhao, Wolf: "Acute and chronic dopamine receptor stimulation modulates AMPA receptor trafficking in nucleus accumbens neurons cocultured with prefrontal cortex neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 16, pp. 4216-30, 2008

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, 2008

Vest, Davies, OLeary, Port, Bayer: "Dual mechanism of a natural CaMKII inhibitor." in: Molecular biology of the cell, Vol. 18, Issue 12, pp. 5024-33, 2007

Background publications Meng, Guo, Zhang, Song, Zhang et al.: "Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia ..." in: Brain research, Vol. 967, Issue 1-2, pp. 161-9, 2003

Gleason, Higashijima, Dallman, Liu, Mandel, Fetcho: "Translocation of CaM kinase II to synaptic sites in vivo." in: Nature neuroscience, Vol. 6, Issue 3, pp. 217-8, 2003

Hudmon, Schulman: "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in cellular function." in: Annual review of biochemistry, Vol. 71, pp. 473-510, 2002

Elgersma, Fedorov, Ikonen, Choi, Elgersma, Carvalho, Giese, Silva: "Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning." in: Neuron, Vol. 36, Issue 3, pp. 493-505, 2002

Soderling: "CaM-kinases: modulators of synaptic plasticity." in: Current opinion in neurobiology, Vol. 10, Issue 3, pp. 375-80, 2000

Picciotto, Czernik, Nairn: "Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site." in: The Journal of biological chemistry, Vol. 268, Issue 35, pp. 26512-21, 1994

Schulman, Hanson: "Multifunctional Ca2+/calmodulin-dependent protein kinase." in: Neurochemical research, Vol. 18, Issue 1, pp. 65-77, 1993