DBH antibody (N-Term)

Catalog No. ABIN372612

The Sheep Polyclonal anti-DBH antibody (ABIN372612) specifically detects DBH in WB and IHC (p). The antibody is reactive with Human and Non-Human Primate samples.

Quick Overview for DBH antibody (N-Term) (ABIN372612)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Human, Non-Human Primate

Host: Sheep

Clonality: Polyclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DBH Antibody

Binding Specificity: N-Term

Specificity: Specific for the ~75k DBH protein in Western blots.

Cross-Reactivity (Details): Species reactivity (tested):Human and non-Human Primates.

Purification: Affinity Chromatography using a Sulfo-Link® column matrix to which the peptide immunogen was coupled.

Immunogen: Peptide from the N-terminal region of human dopamine Beta-hydroxylase (DBH), conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: Dopamine beta-Hydroxylase

Background: DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).Synonyms: DBH, DBM, DOPO, Dopamine beta-monooxygenase

Gene ID: 1621

NCBI Accession: NP_000778

UniProt: P09172

Pathways: Carbohydrate Homeostasis