Glutamate Receptor 1 antibody (pSer845)

Catalog No. ABIN372653

Product Details anti-Glutamate Receptor 1 Antibody

Target: Glutamate Receptor 1 (GLUR1)

Binding Specificity: pSer845

Reactivity: Human, Rat, Mouse, Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~100k GluR1 protein phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is s completely eliminated by treatment with lambda-Ptase.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser845 of GluR1.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunofluorescence. Immunohistochemitsry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term.

References for anti-Glutamate Receptor 1 antibody (ABIN372653)

Shen, Fu, Cheng, Fu, Ip: "Melanocortin-4 receptor regulates hippocampal synaptic plasticity through a protein kinase A-dependent mechanism." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 2, pp. 464-72, (2013) (PubMed).

Santini, Feyder, Gangarossa, Bateup, Greengard, Fisone et al.: "Dopamine- and cAMP-regulated phosphoprotein of 32-kDa (DARPP-32)-dependent activation of extracellular signal-regulated kinase (ERK) and mammalian target of rapamycin complex 1 (mTORC1) signaling in ..." in: The Journal of biological chemistry, Vol. 287, Issue 33, pp. 27806-12, (2012) (PubMed).

Sears, Liu, Narayanan, Sharf, Yeckel, Laubach, Aghajanian, DiLeone: "Regulation of nucleus accumbens activity by the hypothalamic neuropeptide melanin-concentrating hormone." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 24, pp. 8263-73, (2010) (PubMed).

Rebholz, Nishi, Liebscher, Nairn, Flajolet, Greengard: "CK2 negatively regulates Galphas signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 33, pp. 14096-101, (2009) (PubMed).

Santini, Heiman, Greengard, Valjent, Fisone: "Inhibition of mTOR signaling in Parkinson's disease prevents L-DOPA-induced dyskinesia." in: Science signaling, Vol. 2, Issue 80, pp. ra36, (2009) (PubMed).

Zheng, Keifer: "PKA has a critical role in synaptic delivery of GluR1- and GluR4-containing AMPARs during initial stages of acquisition of in vitro classical conditioning." in: Journal of neurophysiology, Vol. 101, Issue 5, pp. 2539-49, (2009) (PubMed).

Meyer, Richer, Benkovic, Hayashi, Kansy, Hale, Moy, Kim, OCallaghan, Tsai, Greengard, Nairn, Cowan, Miller, Antich, Bibb: "Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 47, pp. 18561-6, (2008) (PubMed).

Nishi, Kuroiwa, Miller, OCallaghan, Bateup, Shuto, Sotogaku, Fukuda, Heintz, Greengard, Snyder: "Distinct roles of PDE4 and PDE10A in the regulation of cAMP/PKA signaling in the striatum." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 42, pp. 10460-71, (2008) (PubMed).

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).

Target Details for Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Alternative Name: Glutamate Receptor 1 / GLUR1 (GLUR1 Products)

Synonyms: GLUH1 antibody, GLUR1 antibody, GLURA antibody, GluA1 antibody, HBGR1 antibody, gluR-A antibody, 2900051M01Rik antibody, Glr-1 antibody, Glr1 antibody, GluR-A antibody, GluRA antibody, Glur-1 antibody, Glur1 antibody, HIPA1 antibody, gluR-K1 antibody, GRIA1 antibody, glur1 antibody, Gria1 antibody, glur1b antibody, gria1.2 antibody, sb:eu293 antibody, ZfGluR1a antibody, glur1a antibody, gria1.1 antibody, zfGRIA1[a] antibody, glutamate ionotropic receptor AMPA type subunit 1 antibody, glutamate receptor, ionotropic, AMPA1 (alpha 1) antibody, glutamate receptor, ionotropic, AMPA 1 antibody, glutamate receptor, ionotropic, AMPA 1 L homeolog antibody, GluR1 protein antibody, Glutamate receptor 1 antibody, glutamate receptor, ionotropic, AMPA 1b antibody, glutamate receptor, ionotropic, AMPA 1a antibody, GRIA1 antibody, Gria1 antibody, gria1 antibody, gria1.L antibody, glur1 antibody, glr-1 antibody, gria1b antibody, gria1a antibody

Background: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990, Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993, Mammen et al., 1999, Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory (Soderling and Derkach, 2000).Synonyms: AMPA-selective glutamate receptor 1, AMPA1, GLUH1, GRIA1, GluR-1, GluR-A, GluR-K1, Glutamate receptor ionotropic

Gene ID: 50592

NCBI Accession: NP_113796

UniProt: P19490

Pathways: PI3K-Akt Signaling