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MEK1 antibody (pThr292)
MAP2K1
Reactivity: Human, Mouse, Rat, Dog, Cow, Non-Human Primate
WB
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-MEK1 Antibody
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Target
See all MEK1 (MAP2K1) Antibodies
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Binding Specificity
All epitopes for MEK1 antibodies
pThr292
Reactivity
All reactivities for MEK1 antibodies
Human, Mouse, Rat, Dog, Cow, Non-Human Primate
Host
All hosts for MEK1 antibodies
Rabbit
Clonality
All clonalities for MEK1 antibodies
Polyclonal
Conjugate
All conjugates for MEK1 antibodies
This MEK1 antibody is un-conjugated
Application
All applications for MEK1 antibodies
Western Blotting (WB)
Specificity
This antibody is specific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.
Cross-Reactivity (Details)
Species reactivity (expected):Mouse, Human, Bovine, Canine and non-Human Primates. Species reactivity (tested):Rat.
Purification
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK1.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody undiluted (in aliquots) at-20 °C.
Target Details for MEK1
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Target
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Alternative Name
MAP2K1 (MAP2K1 Products )
Synonyms
Mek1 antibody, CFC3 antibody, MAPKK1 antibody, MEK1 antibody, MKK1 antibody, PRKMK1 antibody, MEKK1 antibody, Prkmk1 antibody, mek-2 antibody, si:ch211-242m18.2 antibody, wu:fj56a12 antibody, wu:fj61b01 antibody, zgc:56557 antibody, ATMEK1 antibody, F20B18.180 antibody, F20B18_180 antibody, MAP kinase/ ERK kinase 1 antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 antibody, NMAPKK antibody, MEK antibody, mitogen activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 L homeolog antibody, MAP kinase/ ERK kinase 1 antibody, Map2k1 antibody, MAP2K1 antibody, map2k1.L antibody, map2k1 antibody, MEK1 antibody
Background
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993, Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991, Seger et al., 1991, Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK1, PRKMK1
Gene ID
5604
NCBI Accession
NP_002746
UniProt
Q02750
Pathways
MAPK Signaling , RTK Signaling , Interferon-gamma Pathway , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , Activation of Innate immune Response , Toll-Like Receptors Cascades , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , BCR Signaling
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