SYT1 antibody (pThr202)

Catalog No. ABIN372724

Product Details anti-SYT1 Antibody

Target: SYT1 (Synaptotagmin I (SYT1))

Binding Specificity: pThr202

Reactivity: Human, Rat, Mouse, Cow, Chicken, Dog, Zebrafish (Danio rerio), Non-Human Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Specificity: This antibody recognizes the ~60k-62k synaptotagmin protein phosphorylated at Thr202 in Western Blots of Rat brain lysate. The immunolabeling is completely eliminated by lambda-phosphatase treatment.

Cross-Reactivity (Details): Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse, non-Human Primate and Zebrafish.
Species reactivity (tested):Rat.

Purification: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr202 of Synaptotagmin.

Isotype: IgG

Application Details

Application Notes: Dot blot: 1/1,000. Western blot: 1/1,000. Immunohistochemistry: 1/400.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: Synaptotagmin-1 (SYT1 Products)

Synonyms: P65 antibody, SVP65 antibody, SYT antibody, CG3139 antibody, D. Syt I antibody, DSYT antibody, DSYT2 antibody, DSypt antibody, DSyt antibody, Dmel\\CG3139 antibody, Droso1 antibody, Dsyt2 antibody, SYT I antibody, SYT1 antibody, Syt antibody, Syt 1 antibody, Syt I antibody, Syt-1 antibody, SytI antibody, dSYT antibody, dsyt1 antibody, dsytI antibody, l(2)23AB1 antibody, l(2)23Ba antibody, l(2)k05909 antibody, syt antibody, syt 1 antibody, syt I antibody, syt-1 antibody, syt1 antibody, sytI antibody, AW124717 antibody, G630098F17Rik antibody, Syti antibody, GB20036 antibody, DKFZP459P193 antibody, svp65 antibody, p65 antibody, wu:fj41c01 antibody, wu:fk56d05 antibody, zgc:92899 antibody, synaptotagmin 1 antibody, Synaptotagmin 1 antibody, synaptotagmin I antibody, synaptotagmin-1 antibody, synaptotagmin, p65 antibody, synaptotagmin Ia antibody, synaptotagmin 1 L homeolog antibody, SYT1 antibody, Syt1 antibody, Tsp_06980 antibody, Tsp_08220 antibody, syt1 antibody, LOC100533340 antibody, syt1a antibody, syt1.L antibody

Background: Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001, Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999, Lee et al., 2004).Synonyms: SYT1, SYT2, SYT3, SYT4, SYT5

Gene ID: 6857

NCBI Accession: NP_001129277

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport