8-Oxoguanine DNA Glycosylase (OGG1) antibody

Antigen: 8-Oxoguanine DNA Glycosylase (OGG1)

Synonyms: HMMH, MUTM, OGH1, HOGG1, OGG1, ogg1, 8-OXOGUANINE-DNA GLYCOSYLASE 1, F8K7.14, F8K7_14, 19.m02329, Tb04.1H19.930

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372767

Additional Information

Characteristics: Synonyms: MMH, MUTM, OGH1, N-glycosylase/DNA lyase

Alternative name: OGG1

Swiss-Prot: O70249

Immunogen: Synthetic peptide (C-HPKTSQAKGPSPLANK) from an Internal region of Mouse OGG1.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Isotype: IgG  (Matching secondary antibodies)

Description: The Ogg1 gene encodes a DNA repair enzyme (N glycosylase/DNA lyase) that incises DNA at8 oxoG residues. N glycosylase/DNA lyase excises 7,8 dihydro 8 oxoguanine and 2,6diamino 4 hydroxy 5 N methylformamidopyrimidine (FAPY) from damaged DNA. Nglycosylase/DNA lyase has a beta lyase activity that nicks DNA 3' to the lesion. Alternative splicing of the C terminal region of the Ogg1 gene classifies splice variants intotwo major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1alternative splice variants end with exon 7 and type 2 end with exon 8. All variants sharethe N terminal region in common. Many alternative splice variants for this gene have beendescribed, but the full length nature for every variant has not been determined. The Nterminus of this gene contains a mitochondrial targetting signal, essential formitochondrial localization.

Specificity: Recognizes 8-oxoguanine DNA Glycosylase Homolog 1 (OGG1). Species Reactivity: Tested: Mouse. Expected from sequence similarity: Rat.

Application Details

Application Notes: ELISA: 1/28000. Western Blot: 0.1-0.3 µg/ml. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris Saline Buffer, pH 7.3 containing 0.02% Sodium Azide as preservativeand 0.5% BSA as stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only