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Antibodies
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anti-Jagged 1 (JAG1) (AA 110-125) antibody
Jagged 1 (JAG1) (AA 110-125) antibody
| Antigen |
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| Synonyms |
AGS, AHD, AWS, HJ1, CD339, JAGL1, MGC104644, Htu, Ozz, Ser-1, slalom, Serrate-1, Headturner, JAG1 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN372790 |
| Quantity |
50 µg (0.53 mg/ml) (Variants) |
| Price |
390.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Jagged-1, hJ1, JAGL1
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Alternative name
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CD339 / JAG1
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Swiss-Prot
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P78504
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Immunogen
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Synthetic peptide corresponding to aa 110-125 of Human Jagged-1 protein.
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Cross-Reactivity
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Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Jagged-1 (JAG1) is a cell surface protein which is one of several ligands that activate Notchand related receptors. JAG1 functions in an embryologically important signaling pathwayand has also been shown to play a role in hematopoiesis. JAG1 inhibits proliferation ofcd34+ macrophage progenitor cells and a JAG1 gene abnormality may be an aggravatingfactor in extrahepatic biliary atresia. Activation of NOTCH1 signaling by JAG1 inducesmonocyte-derived dendritic cell maturation in vitro. JAG1 interaction with NOTCH1 on tumorcells dramatically induces proliferation and inhibition of apoptosis in vitro. Eitherhaploinsufficiency for wild-type JAG1 and/or dominant negative effects produced bymutated JAG1 are responsible for the Alagille syndrome.
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Specificity
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Recognizes Human Jagged-1 (JAG1). Species Reactivity: Tested: Human. Expected from sequence similarity: Chimpanzee, Rat and Mouse (100% homology). Canine(81%) and Xenopus laevis (85%). Add. Information: Predicted Molecular Weight: 134 kDa.
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Application Notes
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ELISA: 1/2500-1/10000. Western Blot: 1/250-1/1000. Detects a band approximately 150kD in size corresponding toJagged-1 in Mouse liver whole cell lysate and a 75kD band in Human brain and kidneylysates. Immunocytochemistry. Immunofluorescence: 1/100-1/500. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.53 mg/ml
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Purification
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Aff - Purified
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Buffer
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PBS containing 0.01% Sodium Azide as preservative.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Jagged 1 (JAG1)", type "Antibodies"
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Hosts
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Mouse (5), Rabbit (4), Goat (1)
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Reactivities
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Human (10), Mouse (Murine) (3), Rat (Rattus) (2), Chimpanzee (1)
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Applications
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Western Blotting (WB) (9), Enzyme Immunoassay (EIA) (7), Immunofluorescence (IF) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), ELISA (1), ELISA (Detection) (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunohistochemistry (IHC) (1)
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Epitopes
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AA 110-125 (1)
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