Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
This antibody recognizes Calcium/Calmodulin-dependent Protein Kinase Type II (CAMK2). Specific for the ~50k a-CaM Kinase II and the ~60k R-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the X-phosphatase treatment.
Cross-Reactivity (Details)
Species reactivity (expected):Human, Mouse, Xenopus. Species reactivity (tested):Rat.
Purification
Immunoaffinity Chromatography
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoThr286 found in Rat brain CaM Kinase II.
CAMK2A
Reactivity: Human
WB, ELISA, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry on paraffin sections: 1/200. Western blot: 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
CAMK2A
(Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))
camk2 antibody, camk2b antibody, zgc:112538 antibody, zgc:123320 antibody, CAMKA antibody, CaMKII antibody, R74975 antibody, mKIAA0968 antibody, PK2CDD antibody, PKCCD antibody, calcium/calmodulin dependent protein kinase II alpha antibody, calcium/calmodulin dependent protein kinase (CaM kinase) II alpha S homeolog antibody, calcium/calmodulin-dependent protein kinase II alpha antibody, CAMK2A antibody, camk2a.S antibody, camk2a antibody, Camk2a antibody
Background
CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/Calmodulin-dependent Protein Kinase Type II, KIAA0968