MTR antibody (C-Term)

Catalog No. ABIN374080

Product Details anti-MTR Antibody

Target: MTR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase (MTR))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This MTR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-VEKWLGPILG YDTD

Specificity: This antibody detects Human MTR at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity C0.02% sodium azide, pH~7.3 with 0.5% bovine serum albuminhromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_000245.2. Genename: MTR

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 150 kDa band seen in HeLa and Jurkat cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MTR

Target: MTR (5-Methyltetrahydrofolate-Homocysteine Methyltransferase (MTR))

Alternative Name: Methionine Synthase / MTR (MTR Products)

Synonyms: HMAG antibody, MS antibody, cblG antibody, AI894170 antibody, D830038K18Rik antibody, methioninesynthase antibody, 5-methyltetrahydrofolate-homocysteine methyltransferase antibody, MTR antibody, Mtr antibody

Background: MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G.Synonyms: 5-methyltetrahydrofolate--homocysteine methyltransferase, Vitamin-B12 dependent methionine synthase

Gene ID: 4548

NCBI Accession: NP_000245

UniProt: Q99707

Pathways: Methionine Biosynthetic Process