FYN antibody (N-Term)

Catalog No. ABIN374105

The Goat Polyclonal anti-FYN antibody is suitable to detect FYN in samples from Human, Mouse, Rat, Cow, Dog and Pig. It has been validated for WB.

Quick Overview for FYN antibody (N-Term) (ABIN374105)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This FYN antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FYN Antibody

Binding Specificity: N-Term

Sequence: VQCKDKEATK LTE

Specificity: This antibody is expected to recognise all three reported isoforms of FYN (NP_002028.1, NP_694592.1 and NP_694593.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_002028.1, NP_694592.1, NP_694593.1. Genename: FYN

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 55-60 kDa band observed in Jurkat, A431 and NIH-3T3lysates (predicted MW of 60 kDa according to NP_002028.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: FYN

Background: Fyn is a membrane-associated, non-receptor protein tyrosine kinase approximately 59 kDa, which belongs to the Src family of cytoplasmic tyrosine kinases. Fyn is very strongly similar to mouse Fyn, v-yes and c-src. Fyn is expressed predominately in tissues of neuronal and hematopoietic origin. Neuronal Fyn and hematopoietic Fyn differ at the junction of the SH2 and kinase domains due to tissue specific alternative splicing. Fyn has been shown to be involved in B cell and T cell activation as well as keratinocyte differentiation. In T cells, Fyn associates with the T-cell antigen receptor and Thy1.Synonyms: Proto-oncogene tyrosine-protein kinase Fyn, Protooncogene Syn, SLK, p59-Fyn

Gene ID: 2534

NCBI Accession: NP_002028

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling