PPP2R5B antibody (C-Term)

Catalog No. ABIN374135

The Goat Polyclonal anti-PPP2R5B antibody is suitable to detect PPP2R5B in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for PPP2R5B antibody (C-Term) (ABIN374135)

Target: PPP2R5B (Protein Phosphatase 2, Regulatory Subunit B', beta (PPP2R5B))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R5B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPP2R5B Antibody

Binding Specificity: C-Term

Sequence: C-QRLTPQVAAS GGQS

Specificity: This antibody detects PPP2R5B at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006235.1 Genename: PPP2R5B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 2000. Western Blot: Recommended concentration: 2-3 μg/mL. Approx.55 kDa band observed inHuman Brain (Cerebellum) lysates (calculated MW of 57.5 kDa according to NP_006235.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for PPP2R5B

Target: PPP2R5B (Protein Phosphatase 2, Regulatory Subunit B', beta (PPP2R5B))

Alternative Name: PPP2R5B

Background: Synonyms: PP2A B subunit B' beta isoform, PP2A B subunit B56 beta isoform, PR61 beta isoform, R5 beta isoform, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, protein phosphatase 2A regulatory subunit B' beta

Gene ID: 5526

NCBI Accession: NP_006235

UniProt: Q15173

Pathways: PI3K-Akt Signaling, ER-Nucleus Signaling