Annexin A2 antibody (N-Term)

Catalog No. ABIN374164

Product Details anti-Annexin A2 Antibody

Target: Annexin A2 (ANXA2)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: STVHEILCKL SLEGD

Specificity: This antibody is expected to recognize both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow, and Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_001002858.1, NP_004030.1, NP_001002857.1, NP_001129487.1. Genename: ANXA2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell lines A431 andA549 (calculated MW of 38.6 kDa according to NP_004030.1 and NP_001002857.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: Annexin A2 / ANXA2 (ANXA2 Products)

Synonyms: ANX2 antibody, ANX2L4 antibody, CAL1H antibody, LIP2 antibody, LPC2 antibody, LPC2D antibody, P36 antibody, PAP-IV antibody, AW215814 antibody, Cal1h antibody, p36 antibody, anx2 antibody, lip2 antibody, lpc2 antibody, cal1h antibody, lpc2d antibody, pap-i antibody, anx2l4 antibody, MGC76145 antibody, ANXA2 antibody, anxa2 antibody, anxa2-a antibody, anxa2-b antibody, annexin A2 antibody, annexin A2 S homeolog antibody, ANXA2 antibody, Anxa2 antibody, anxa2 antibody, anxa2.S antibody

Background: The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: ANX2, ANX2L4, Annexin II, Annexin-2, CAL1H, Calpactin I heavy chain, Chromobindin-8, LPC2D, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36

Gene ID: 302

NCBI Accession: NP_001002858

UniProt: P07355

Pathways: S100 Proteins