Annexin A2 antibody (N-Term)

Catalog No. ABIN374164

This Goat Polyclonal antibody specifically detects Annexin A2 in WB. It exhibits reactivity toward Human, Mouse, Rat, Cow and Pig.

Quick Overview for Annexin A2 antibody (N-Term) (ABIN374164)

Target: Annexin A2 (ANXA2)

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Annexin A2 Antibody

Binding Specificity: N-Term

Sequence: STVHEILCKL SLEGD

Specificity: This antibody is expected to recognize both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow, and Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_001002858.1, NP_004030.1, NP_001002857.1, NP_001129487.1. Genename: ANXA2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 4000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell lines A431 andA549 (calculated MW of 38.6 kDa according to NP_004030.1 and NP_001002857.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: Annexin A2 / ANXA2

Background: The Annexins are a family of structurally similar proteins. Annexins bind to phospholipids and may be involved in regulation of membrane transport, membrane channel activity, and interaction of the cell membrane with the extracellular matrix. Annexin II is a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Synonyms: ANX2, ANX2L4, Annexin II, Annexin-2, CAL1H, Calpactin I heavy chain, Chromobindin-8, LPC2D, Lipocortin II, Placental anticoagulant protein IV, Protein I, p36

Gene ID: 302

NCBI Accession: NP_001002858

UniProt: P07355

Pathways: S100 Proteins