APEX1 antibody (N-Term)

Catalog No. ABIN374178

The Goat Polyclonal anti-APEX1 antibody (ABIN374178) specifically detects APEX1 in WB, IHC (p) and EIA. The antibody is reactive with Human, Cow, Pig and Dog samples.

Quick Overview for APEX1 antibody (N-Term) (ABIN374178)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-APEX1 Antibody

Binding Specificity: N-Term

Sequence: PKRGKKGAVA EDGD-C

Specificity: This antibody detects APEX1 / REF-1 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from the N-Terminus of Human APEX1 / APE1 (NP_001632.2). Genename: APEX1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 35 kDa band observed in Human epidermoid fibroblastA431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.5 kDaaccording to NP_001632.2, NP_542379.1, NP_542380.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 / REF-1

Background: Apurinic / apyrimidinic endonuclease (APE1 or REF1) is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. Alterations in APE1 levels have been described in some cancers. APE1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.Synonyms: APE, APEN, APEX, APEX nuclease, APX, Apurinic-apyrimidinic endonuclease 1, HAP1, Protein REF-1, REF1

Gene ID: 328

NCBI Accession: NP_001632

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus