Flotillin 2 antibody (C-Term)

Catalog No. ABIN374221

Product Details anti-Flotillin 2 Antibody

Target: Flotillin 2 (FLOT2)

Binding Specificity: C-Term

Reactivity: Human, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Flotillin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-SKIPLIKKAT GVQV

Specificity: This antibody detects Flotillin-2 (FLOT2) at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004466.2 Genename: FLOT2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 48 kDa band observed in Human Brain (Cerebellum),Lung and Testis lysates (calculated MW of 47.8 kDa according to NP_004466.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Flotillin 2

Target: Flotillin 2 (FLOT2)

Alternative Name: Flotillin-2 / FLOT2 (FLOT2 Products)

Synonyms: ECS-1 antibody, ECS1 antibody, ESA antibody, ESA1 antibody, M17S1 antibody, AI573412 antibody, Esa antibody, reggie-2 antibody, CG11547 antibody, CG14409 antibody, CG32593 antibody, DmFlo2 antibody, Dmel\\CG32593 antibody, FLO2 antibody, FLO[Dm]-2 antibody, Flo2 antibody, flo2 antibody, reggie-1 antibody, ecs-1 antibody, ecs1 antibody, esa antibody, esa1 antibody, flotillin-2 antibody, FLOT2 antibody, fb48a04 antibody, im:7143869 antibody, wu:fb48a04 antibody, zgc:55821 antibody, flotillin 2 antibody, Flotillin 2 antibody, flotillin 2 L homeolog antibody, flotillin 2a antibody, FLOT2 antibody, Flot2 antibody, Flo2 antibody, flot2.L antibody, flot2 antibody, flot2a antibody

Background: Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. Flotillin 2 may act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.Synonyms: ESA, ESA1, Epidermal surface antigen, M17S1, REG-1, Reg1, Reggie-1

Gene ID: 2319

NCBI Accession: NP_004466

UniProt: Q14254