AIRE antibody (C-Term)

Catalog No. ABIN374235

Product Details anti-AIRE Antibody

Target: AIRE (Autoimmune Regulator (AIRE))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Chromatin Immunoprecipitation (ChIP)

Sequence: C-QSMARPAAPF PS

Specificity: This antibody detects AIRE / APECED at C-term. Expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_000374.1, NP_000649.1. Genename: AIRE

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.01-0.03 μg/mL. Appr.60 kDa in transfected HEK293 lysates (calculated MWof 57.0 kDa according to NP_000374.1). Immunohistochemistry: 2.5 μg/mL. In paraffin embedded Human Thymic medula showsstaining of select nuclei.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE / APECED (AIRE Products)

Synonyms: AIRE1 antibody, APECED antibody, APS1 antibody, APSI antibody, PGA1 antibody, autoimmune regulator antibody, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, AaeL_AAEL013794 antibody, CpipJ_CPIJ001208 antibody, CpipJ_CPIJ001209 antibody, AIRE antibody, Aire antibody

Background: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

Gene ID: 326

NCBI Accession: NP_000374

UniProt: O43918

Pathways: Chromatin Binding